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Improved antioxidant effect of idebenone-loaded polyethyl-2-cyanoacrylate nanocapsules tested on human fibroblasts

Abstract

The protective antioxidant role of idebenone both as free drug and drug-loaded Tween 80-coated polyethyl-2-cyanoacrylate (PECA) nanocapsules is reported. The relationship between oxidative damage and apoptotic or nonapoptotic cell death is evaluated in vitro. Idebenone-loaded nanocapsules were prepd. with the interfacial polymn. method in the presence of Tween 80. Human nonimmortalized fibroblasts, under different stress conditions, either 0.5 mM diethylmaleate (DEM) for 60 min or 0.1 mM H2O2 for 30 min, were used as the exptl. in vitro model. The prodn. of reactive oxygen species, the cell viability, and the nuclear DNA damage were evaluated. The presence of apoptotic damage was evaluated both by the detn. of caspase-3-like protein activity and by Promega's fluorescent apoptotic detection system. DEM and H2O2 affected the cultured cells in different ways. DEM induced a moderate cellular insult, which was efficaciously antagonized by idebenone-loaded PECA nanocapsules. H2O2 elicited severe damage to nuclear DNA, which was reduced by idebenone-loaded PECA nanocapsules. The free drug was less effective than idebenone-loaded nanocapsules. The findings reported here demonstrate that an improved antioxidant effect was obtained with a low idebenone concn. (0.5 mM) when the drug was entrapped within Tween 80-coated PECA nanocapsules.

Autori

PUGLISI - Giovanni

Altri Autori

Palumbo Maddalena
Russo Alessandra
Cardile Venera
Renis Marcella
Paolino Donatella
Fresta Massimo