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Ethanol-induced injury in rat primary cortical astrocytes involves oxidative stress: effect of idebenone.

Abstract

Ethanol-induced neurol. disorders have recently been characterized. Indeed, evidence has been collected indicating that chronic ethanol consumption leads to direct or indirect changes in the viability of central nervous system cells. Here we investigated the role of free radical overprodn. in primary cortical rat astroglial cells undergoing chronic treatment with ethanol (100 mM). In particular, exposure of astroglial cell cultures to ethanol for 12 consecutive days produced an increased release of lactic dehydrogenase, a decrease on glutamine synthase activity being both effects accompanied by decrease in astroglial viability as detected by MTT (Thiazolyl Blue) test. These effects were accompanied by an increased formation of malondialdehyde (a marker of lipid peroxidn.) and by abnormal formation of heat shock protein, being both effects antagonized by liposomally entrapped idebenone, a non-peptidyl free radical scavenger. Taken together, these results suggest that ethanol-induced injury on astroglial cells are mediated by abnormal formation of free radical species and this may represent a useful approach in the treatment of ethanol-related brain disorders.

Autori

PUGLISI - Giovanni

Altri Autori

Muscoli Carolina
Fresta Massimo
Cardile Venera
Palumbo Maddalena
Renis Marcella
Paolino Donatella
Nistico Steven
Rotiroti Domenicantonio
Mollace Vincenzo