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Phospholipid vesicles as biosensors to evaluate antimicrobial agent-membrane interaction

Abstract

A review and discussion. The use of phospholipid membranes as biosensors to evaluate both qual. and quant. the interaction of antimicrobial agent with the bilayer structure is reported. The binding of antimicrobial agent to the membrane model is relevant to an understanding of drug diffusion across phospholipid bilayers as one component of uptake into cells. It has been demonstrated that membrane binding is the initial step in diffusion. Here the authors present the importance of the microspeciation physico-chem. properties of antibacterial agents in the detn. of intracellular accumulation and membrane permeation pathways of some antibacterial classes, such as fluoroquinolones, diaminobenzylpyrimidines and peptides. Phospholipid biosensors allow the study and clarification of the equil. distribution of antimicrobial agents, as an important factor in modulating efficacy. Phospholipid biosensors are also a very useful device to clarify the mechanisms involved in the toxicity of some drugs, i.e. aminoglycosides and macrolides. The prepn. and characterization of phospholipid membranes as biosensor devices are also reported

Autori

PUGLISI - Giovanni

Altri Autori

Fresta Massimo