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Effect of application time of betamethasone 17-valerate 0.1% cream on skin blanching and stratum corneum drug concentration

Abstract

The effect of application time on skin blanching response and stratum corneum concentration after topical application of 0.1% betamethasone-17-valerate cream on healthy volunteers was assessed. Total corticosteroid content in the stratum corneum (SC) was determied at different application times (0.5, 8, 10 and 24 h) from 5 subjects in whom blanching was also evaluated at the same application times by visual score, colorimetry (L-, a- and b-values) and Laser Doppler flowmetry. Significant differences between the blanching response at 0.5 h and the other time points (8, 10 and 24 h) were observed by visual assessment and a-value readings from a chromameter. However, at 24 h visual score and Da-values were lower than those measured at 8 and 10 h. but this difference was significant only for Da-values. This findings suggest that skin blanching may not be related to drug concentration. in the stratum corneum and that Da-readings may be more sensitive and accurate than visual score in evaluating skin blanching. L-values were not significantly different at all the time points while b-values at 0.5 h were significantly different only from those at 8 and 10 h. Skin blanching was not observed when laser Doppler flow was measured while the concentration parameter was capable of detecting blanching; however, the concentration values were not significantly different at all the application times. The duration of application should be carefully chosen to assess betamethasone 17-valerate topical bioavailability since after long application time skin blanching response may not be related to drug concentration in the stratum corneum

Autori

MONTENEGRO - Lucia

Altri Autori

Bonina Francesco Paolo, Ademola John, Maibach Howard.